In a Yeast Two-Hybrid Analysis of Several Strains Containing Different $\beta$

question 2

Multiple Choice

In a yeast two-hybrid analysis of several strains containing different prey/bait combinations, the screening of interacting proteins is done by growing cells on a medium containing X-Gal. White and blue colonies are observed after incubation. Which of the following statements is NOT true regarding blue/white selection in the yeast two-hybrid method?

• A) Blue colonies result from X-Gal hydrolyzed by the reporter enzyme \(\beta\)-galactosidase.
• B) White colonies result from cells in which the prey and bait proteins do not interact.
• C) Galactose released from X-Gal hydrolysis does not contribute to a colony's blue color.
• D) White colonies indicate that \(\beta\)-galactosidase catalyzed the hydrolysis of X-Gal.
• E) Blue colonies are an indication that GAL4 activated transcription of GAL1-lacZ.

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Definitions:

Quantity Supplied

The total amount of a particular good or service that producers are willing and able to sell at a given price over a specified period.

Quantity Demanded

The amount of a good or service that consumers are willing and able to buy at a specific price.

Surplus

The situation in which the quantity of goods or services supplied exceeds the quantity demanded at the current price.

Quantity Supplied

the amount of a good or service that producers are willing and able to sell at a given price over a specific period.