In a Yeast Two-Hybrid Analysis of Several Strains Containing Different $\beta$

question 2

Multiple Choice

In a yeast two-hybrid analysis of several strains containing different prey/bait combinations, the screening of interacting proteins is done by growing cells on a medium containing X-Gal. White and blue colonies are observed after incubation. Which of the following statements is NOT true regarding blue/white selection in the yeast two-hybrid method?

• A) Blue colonies result from X-Gal hydrolyzed by the reporter enzyme \(\beta\)-galactosidase.
• B) White colonies result from cells in which the prey and bait proteins do not interact.
• C) Galactose released from X-Gal hydrolysis does not contribute to a colony's blue color.
• D) White colonies indicate that \(\beta\)-galactosidase catalyzed the hydrolysis of X-Gal.
• E) Blue colonies are an indication that GAL4 activated transcription of GAL1-lacZ.

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Definitions:

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Luke Skywalker

A fictional character from the "Star Wars" universe, known as a Jedi who plays a pivotal role in the defeat of the Galactic Empire.

Fallacy of Context

An error in reasoning where the context necessary to understand or properly apply an argument is ignored or misrepresented.

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