Why Must Double-Stranded DNA Probes Be Denatured When Used in Northern

question 68

Multiple Choice

Why must double-stranded DNA probes be denatured when used in northern blot experiments?

• A) to ensure that they are properly radioactively labeled or tagged with a dye
• B) to ensure that the DNA coding strand hybridizes to RNA on the membrane
• C) to ensure that both DNA strands bind the single-stranded RNA on the membrane
• D) all of the above
• E) none of the above