Capillary Columns Require Conditioning Prior to Their First Use

question 19

Multiple Choice

Capillary columns require conditioning prior to their first use.Typical conditioning is washing with 1 M NaOH for 1 hour,followed by 1 hour of water,then 1 hour of 6 M HCl,and finally 1 hour of run buffer.Which statements are INCORRECT?
I The NaOH wash is thought to generate the Si-OH groups on the silica surface.
II The NaOH wash removes any acidic contaminants from the silica surface.
III The HCl wash is thought to generate the Si-OH groups on the silica surface.
IV The HCl wash removes metal ions from the capillary surface.

• A)I and III
• B)I and IV
• C)II and III
• D)II and IV
• E)I,II,and III

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