You Are Growing a Population of Cells That Appear to Be

question 39

Multiple Choice

You are growing a population of cells that appear to be resistant to G418,a chemical that is normally toxic to cells.Upon further analysis of the cells' genome you find that it contains a genomic sequence that encodes the protein neomycin phosphotransferase.What would you do to test if this protein confers resistance to the G418 chemical?

• A)determine if the cells are also making β-galactosidase
• B)tag the protein with green fluorescent protein to see if it is degraded by G418
• C)label a fragment of the gene to see where it is expressed
• D)clone the gene into a G418-sensitive cell line,then treat these cells with G418

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Definitions:

Consideration

In contract law, a benefit which must be bargained for between the parties, and is the essential reason for a party entering into a contract.

Paid in Full

A declaration that all outstanding debts or obligations have been completely settled.

Liable

Legally responsible or obligated to do something, particularly paying compensation for harm or damage.

Requirements Contract

A contract in which one party agrees to purchase all of its required goods or services from the other party.