Fluorescence Microscopy of Cells That Are Labeled with a Green

question 3

Multiple Choice

Fluorescence microscopy of cells that are labeled with a green fluorescent antibody against microtubules (a cytoskeletal component) differs from cells expressing green fluorescent protein (GFP) tagged to beta tubulin (a component of microtubules) in all the following ways EXCEPT:

• A)the antibody labeled cells need to be permeabilized before visualization,while the GFP cells do not.
• B)the microtubules could change their localization over time in the GFP cells,but not in the antibody labeled cells.
• C)the GFP cells do not need to be treated with fixatives,but the antibody labeled cells do.
• D)the antibody labeled cells will identify the microtubule structures,while the GFP will be observed in other places in the cell because it is not as specific as an antibody.

Understand the environmental conditions favoring fungal growth.

Identify characteristics distinguishing coenocytic and septate hyphae.

Discuss the interactions between fungi and other organisms, including parasitism and symbiosis.

Describe strategies for combating fungal parasites in aquaculture.

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Definitions:

Manipulated

Altered or controlled in a skillful or sometimes deceitful manner to achieve a desired outcome.

Behavior

The actions or reactions of an organism, often in relation to its environment, which can be observed and measured.

Littering

The improper disposal of waste products in an inappropriate place, typically in public areas.

Solitary Confinement

A form of imprisonment where an inmate is isolated from any human contact, except for members of the prison staff.