You Are Studying Lamins and Use an Antibody Method to Follow

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The Answer is Normally,antibody labeling can be used in tandem with electron microscopy to visualize proteins in thin sections.Cells are lightly fixed to avoid denaturing the epitopes on the desired protein and,after freezing,the tissue is sectioned at very low temperature.The tissue is thawed and a specific antibody to the desired protein is applied as in immunofluorescence microscopy.To detect the antibody,however,the tissue is incubated with electron-dense gold particles attached to protein A,a bacterial protein that binds the Fc segment of all antibodies.These gold particles are seen at the level of the electron microscope;however,you would not be able to see lamins in E.coli because bacteria do not contain membrane-bounded organelles and lamins are a component of the nuclear envelope.

You Are Studying Lamins and Use an Antibody Method to Follow

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