Fluorescence Microscopy of Cells That Are Labeled with a Green

question 3

Multiple Choice

Fluorescence microscopy of cells that are labeled with a green fluorescent antibody against microtubules (a cytoskeletal component) differs from cells expressing green fluorescent protein (GFP) tagged to beta tubulin (a component of microtubules) in all the following ways EXCEPT:

• A)the antibody labeled cells need to be permeabilized before visualization,while the GFP cells do not.
• B)the microtubules could change their localization over time in the GFP cells,but not in the antibody labeled cells.
• C)the GFP cells do not need to be treated with fixatives,but the antibody labeled cells do.
• D)the antibody labeled cells will identify the microtubule structures,while the GFP will be observed in other places in the cell because it is not as specific as an antibody.

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