How Can Somatic Cell Nuclear Transfer Be Used to Clone

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The Answer is Although a relatively inefficient method of cloning,as evidenced from the data that emerged following cloning Dolly the sheep,the technique relies on removing the nucleus from a fully differentiated cell and transplanting it into an enucleated egg of a donor mouse.In one example,the nucleus from an olfactory sensory neuron of a mouse that expresses green fluorescent protein (GFP)was transplanted into an enucleated egg and then differentiated in culture until the inner cell mass stage.GFP-expressing ES cells were isolated and injected into a tetraploid blastocyst host.When this chimeric blastocyst was transplanted into the uterus of a pseudopregnant female,the tetraploid cells formed the placenta and the GFP-expressing cells the embryo proper.If all cells express GFP when the pups were born,this would indicate that their nuclei had been derived from that originally isolated from the GFP-expressing olfactory neuron.

How Can Somatic Cell Nuclear Transfer Be Used to Clone

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