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You Are Working in a Lab Where You Are Studying

question 34

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You are working in a lab where you are studying a disease that is known to be caused by a single nucleotide change, although the effect this change ultimately has on the protein's structure/function is unknown.You have DNA samples from multiple patients that you suspect of having this disease.What is the most efficient way to test the samples for the relevant mutation?


Definitions:

Control

In the context of experiments, a standard condition or treatment that does not change, used as a benchmark to measure the effects of the experimental variable.

Activation Energies

The minimum amount of energy required to initiate a chemical reaction.

Antibonding π Molecular Orbital

A molecular orbital formed when lobes of p orbitals on two atoms combine in a phase mismatch, weakening the bond between the atoms.

Allyl Cation

A positively charged species (ion), specifically a reactive intermediate with the structure CH2=CH-CH2+.

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