Why Does a Genomic Library Need More Clones Than What

question 32

Multiple Choice

Why does a genomic library need more clones than what would be estimated by calculations to be complete?

• A)There is a significant probability that some sequences would not be represented in a library if it was only the calculated size
• B)The restrriction enzyme that is used to prepare the DNA for cloning will not digest certain sequences within the DNA.
• C)The restriction enzyme used to prepare the DNA for cloning sometimes leaves a different sticky end
• D)Restriction enzymes cleave DNA preferentially at the ends of open reading frames so coding regions are prefereentially cloned.

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Definitions:

Total Return

A measure of the overall return on an investment, including both capital appreciation and dividends or interest, over a specific period.

Rate Of Return

The net gain or loss of an investment over a specified time period, expressed as a percentage of the investment's initial cost.

Combined Effect

The cumulative impact of multiple factors or actions taken together, rather than individually.

Income Yield

The income return on an investment, expressed as a percentage of the investment's current market value.