Primers Are Used in Both PCR and in DNA Sequencing

question 53

Multiple Choice

Primers are used in both PCR and in DNA sequencing.What is the purpose of the primers?

• A)to provide a 3'- OH for DNA polymerase to build a new DNA strand
• B)to terminate synthesis of the growing DNA strand
• C)to add nucleotides to a growing DNA strand
• D)to separate the DNA template strands
• E)to separate various-sized DNA products

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Definitions:

Primary Oocyte

A primary oocyte is an immature egg cell that has begun meiosis but is arrested in its first meiotic division until ovulation.

Metaphase II

A stage of meiosis II where sister chromatids align at the equatorial plate of the cell, preparing for separation into daughter cells.

Prophase I

The first stage of meiosis I during which chromosomes condense and homologous chromosomes pair up.

Mature Follicle

A mature follicle is a fluid-filled sac in the ovary that contains a mature egg ready for ovulation.