A Graduate Student Wants to Clone a Particular Gene into a Plasmid.The

question 4

Multiple Choice

A graduate student wants to clone a particular gene into a plasmid.The sequence includes AluI and BamHI sites on both sides of the desired fragment.AluI cuts symmetrically directly between the G and C nucleotides in a palindromic 5' AGCT 3' sequence.BamHI cuts asymmetrically directly between the G and G nucleotides in a palindromic5' GGATCC 3' sequence.Which of the two restriction endonucleases should the graduate student choose,and why?

• A)BamHI to cut both sides-since it cuts asymmetrically,it'll leave the sticky,cohesive single-strand DNA ends that will make it easier to ligate into a BamHI-cut plasmid DNA sequence.
• B)AluI to cut both sides-it's always easier to ligate together blunt ends of DNA.She should also use AluI on the plasmid she wants to put the fragment into.
• C)BamHI on the fragment,and AluI on the plasmid-this will give her the matching sequences to anneal/ligate together on the fragment/plasmid combination.
• D)BamHI on one side of the fragment,and AluI on the other side-this would keep the fragment from sticking right back to where it was cut out from in the original DNA.

Acknowledge the importance of focusing on customers' needs and jobs in creating successful CVPs.

Differentiate between various approaches to creating CVPs and their effectiveness.

Understand the role of disruption in creating and defining new markets or transforming existing ones.

Understand the importance of sample size in data visualization.

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Definitions:

Labor Union Members

Individuals who are part of an organization that represents workers' interests in negotiations with employers regarding wages, work conditions, and rights.

Collective Bargaining

A process of negotiation between employers and a group of employees aimed at reaching agreements to regulate working salaries, working conditions, benefits, and other aspects of workers' compensation and rights.

Escalator Clauses

Provisions in a contract that allow for an automatic increase in wages or prices under certain conditions.

Productivity Increases

An improvement in the efficiency of production, often measured by the amount of output per unit of input over a period.