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A toxicology screen is set up using thin layer chromatographic plates.Four positive controls/standards are used with the following distance of migration from application point results: Std A = 8 cm; Std B = 15 cm; Std C = 22.The acetone solvent front moved 35 cm.After processing an unknown sample,a solute had a migration distance of 16 cm.What is the Rf of the unknown solute?
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