In a Noncompetitive Immunoassay Reaction Format,a Very High Concentration of Analyte

question 20

Multiple Choice

In a noncompetitive immunoassay reaction format,a very high concentration of analyte antigen in the unknown sample might produce a falsely:

• A) decreased result because the high concentration of antigen binds both the capture and labeled antibody,reducing the number of complexes formed.
• B) increased result because the rate of the forward reaction of antigen-antibody binding is too fast,leading to a high equilibrium constant.
• C) decreased result because of the free excess antigen-binding all antibody sites and not allowing any unlabeled antigen to bind.
• D) increased result because the second labeled antibody will be able to react with the substrate by itself since excess antigen will interfere with any binding.

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