TRIS {(HOCH₂)₃CNH₂} Is One of the Most Common Buffers Used

question 46

Multiple Choice

TRIS {(HOCH₂) ₃CNH₂} is one of the most common buffers used in biochemistry.A solution is prepared by adding enough TRIS and 12 M HCl(aq) to give 1.00 L of solution with [TRIS] = 0.30 M and [TRISH⁺] = 0.60 M.What is the pH of this buffered system if the pK_b is 5.92?

• A)5.92
• B)6.22
• C)7.78
• D)8.08

---

Definitions:

Assembly Line

A manufacturing process in which parts are added to a product in a sequential manner to create a finished product much faster than with individual craftsmanship.