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From One F+ strain,three Distinct Hfr Strains Were Derived

question 39

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From one F+ strain,three distinct Hfr strains were derived.The first three markers transferred during an Hfr X F- cross (different for each of the three Hfr) are:
Hfr 1: ...D A →
Hfr 2: ...E B →
Hfr 3: ...E C D →
Which of the following must be the order of the genes on the bacterial chromosomal circle? (A is shown at both ends to represent circularity.Assume that the Hfr picks up all intermediates between any two represented genes.)


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