Restriction Endonucleases BamH1,NheI,XbaI,and EcoRI Make Sequence-Specific Cuts in DNA at the Following

question 26

Essay

Restriction endonucleases BamH1,NheI,XbaI,and EcoRI make sequence-specific cuts in DNA at the following sequences:
BamHI: $\quad$ 5'-GGATCC-3'
NheI: $\quad$ $\quad$ 5'-GCTAGC-3'
XbaI: $\quad$ $\quad$ 5'-TCTAGA-3'
EcoRI: $\quad$ $\quad$ 5'-GAATTC-3'
All these enzymes break a phosphodiester bond between the first and second nucleotide (counting from the 5' end),leaving a 5' P and a 3' OH.
Could ligase covalently join two ends produced by the following enzymes? If so,would ligation regenerate one of the above restriction sites?
(1)two BamH1 ends?
(2)two XbaI ends?
(3)a BamH1 and an EcoRI end?
(4)an XbaI and an NheI end?
(5)a BamH1 and an NheI end?

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Definitions:

Attribution Theory

The theory of how people make attributions.

Primacy Effect

Tendency to remember information at the beginning of a body of information better than the information that follows.

Cognitive Dissonance

A psychological theory that suggests discomfort arises when a person holds conflicting beliefs, attitudes, or values, often leading to changes in one of the beliefs or behaviors.

Primacy Effect

The tendency to remember information at the beginning of a list better than the information that follows.