Which of the Following About the Sanger Sequencing Method Is/are

question 25

Multiple Choice

Which of the following about the Sanger sequencing method is/are TRUE?

• A)Sanger sequencing uses dideoxynucleotide triphosphates to terminate DNA polymerization.
• B)Dideoxynucleotides lack 2′ and 3′ OH groups in the ribose sugar.
• C)All four deoxynucleotide triphosphates are used in a Sanger sequencing reaction.
• D)Fluorescent dyes can be used to automate Sanger sequencing.
• E)All of the answer options are correct.

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