The autoradiogram shown was generated by the dideoxy sequencing method.Define the DNA sequence of the template strand that was used in this sequencing reaction,noting the 5′ and 3′ ends of this template strand. 
Definitions:
Protist Structures
The varied and complex cellular organizations found in protists, ranging from single-celled to multicellular forms.
Eyespot
A pigmented organelle found in some algae and protozoa, allowing the organism to sense light direction.
Giardiasis
An infection of the small intestine caused by the protozoan parasite Giardia lamblia, leading to diarrhea, abdominal pain, and weight loss.
Diplomonads
A group of flagellated protozoa, often parasites, with two nuclei and multiple flagella, found in anaerobic environments.
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