A Partial Peptide Sequence for the Wild Type and Three $\text { Wild type:\quad met \quad ile \quad arg \quad met \quad asp \quad lys \quad trp... }$

question 18

Essay

A partial peptide sequence for the wild type and three mutant alleles of a gene,PET1,are shown below.Each mutant was caused by a single point mutation.

$\text { Wild type:\quad met \quad ile \quad arg \quad met \quad asp \quad lys \quad trp... }$
$\text { Mutant 1:\quad met \quad ile \quad gln \quad asn \quad gly ... }$
$\text {Mutant 2:\quad met \quad ile \quad arg \quad met \quad gly \quad lys \quad trp...}$
$\text {Mutant 3: \quad met \quad ile \quad ser \quad met \quad asp \quad lys \quad trp...}$

a)Using the amino acid sequence of the wild type and the three mutants,deduce the exact DNA sequence of the coding strand of the wild-type allele.
b)What type of mutation (transition vs.transversion vs.indel;missense vs.nonsense vs.frameshift)has occurred in each mutant?
c)What consequence will each of the above-mentioned DNA mutations have on protein function?

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