Ion Exchange Chromatography Is a Commonly Used Method for Separation

question 68

Essay

Ion exchange chromatography is a commonly used method for separation of biomolecules.
a.What are the two types of ion exchange chromatography?
b.In what order would Arg,Val,and Glu elute from a carboxymethyl column at pH 6.0.Carboxymethyl is negatively charged at pH 6.0.
c.You are trying to purify a protein using ion exchange chromatography.Unfortunately,your protein remains bound to the ion exchange column or it is eluting very slowly.What are the two changes you can make,to try to elute your protein more quickly from this column?

Grasp the significance of physical environments and processes on the Earth and outer space in the formation of organic molecules and life.

Understand the process of endosymbiosis and its genetic implications for eukaryotic evolution.

Understand the concept of Hardy-Weinberg equilibrium and the conditions under which it is maintained.

Recognize and explain the significance of multiple allele systems and balanced polymorphisms in populations.

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