Why Must a Shuttle Vector Possess Two Origins of Replication

question 11

Multiple Choice

Why must a shuttle vector possess two origins of replication instead of one?

• A)Shuttle vectors must replicate in multiple hosts with each host requiring a different origin.
• B)Shuttle vectors must be able to replicate from two different origins of replication depending upon the specific growth conditions.
• C)Shuttle vectors only have one origin of replication,not two.
• D)Shuttle vectors use one origin for replication and the other origin for shuttling recombinant DNA into new hosts.

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