An Outbreak of Salmonella Has Occurred in the Community

question 30

Multiple Choice

An outbreak of Salmonella has occurred in the community. Molecular-based assays will be performed to determine if multiple isolates of Salmonella taken at hospital X are related to each other and part of the outbreak. Laboratory technologists have accumulated 10 different cultures growing Salmonella and suspect that there may be more isolated in the next few days. How should these organisms be handled until the molecular-based assays can be performed in about 3 days?

• A) Maintain organisms in culture growing on solid agar in an incubator 35^o-37^oC.
• B) Freeze suspensions of the organisms at -20^oC.
• C) Isolate DNA from all organisms and store at -20^oC.
• D) Isolate DNA from all organisms and store at 22^o-25^oC.

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