In a Complement-Dependent Cytotoxicity Assay, Lymphocytes Were Incubated in the Presence

question 13

Multiple Choice

In a complement-dependent cytotoxicity assay, lymphocytes were incubated in the presence of antibody of known HLA specificity. Complement was added, and the cells absorbed trypan blue. What is the interpretation of this assay?

• A) The lymphocytes express antigens specific for the antibodies in the assay.
• B) The lymphocytes do not have antigens specific for the antibodies in the assay.
• C) This is not a valid method for identifying HLA antigens.
• D) The cells are resistant to complement lysis.

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