A Patient Is Being Tested to Determine Whether He Has

question 25

Multiple Choice

A patient is being tested to determine whether he has the factor V Leiden mutation by PCR-RFLP analysis. PCR is performed on the patient's DNA and cut with HindIII. The mutation-positive control sample had a single band of expected size, and the mutation-negative control sample had a slightly larger band. The patient's specimen had two bands, one that was the same size as the mutation-positive control band and the other the same as the mutation-negative control. Which of the following is the correct interpretation of these results?

• A) The patient does not have the factor V Leiden mutation.
• B) The patient is heterozygous for the factor V Leiden mutation.
• C) The patient is homozygous for the factor V Leiden mutation.
• D) The results are not valid, and the assay should be repeated.

---

Definitions:

Well-being

The state of being comfortable, healthy, or happy, encompassing physical, mental, and emotional health.

Menopause

The natural cessation of menstruation that typically occurs in women in their late 40s to 50s, marking the end of reproductive ability.

Progesterone

A steroid hormone produced in the ovaries, placenta, and adrenal glands that plays key roles in the menstrual cycle, maintaining pregnancy, and regulating various bodily functions.

Periods

Regular intervals of time, or the cyclical shedding of the uterine lining in females, marking the menstrual cycle.