Patient DNA Has Been Mixed in Separate Wells with a Probe

question 5

Multiple Choice

Patient DNA has been mixed in separate wells with a probe that detects the 1691G>A mutation associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is added to all wells, and the fluorescence is measured in each well. Fluorescence was detected in the well that included the normal probe, and no fluorescence was detected in the well containing the mutant probe. Which of the following assays was performed?

• A) Invader assay
• B) Nonisotopic RNase cleavage assay
• C) Chemical cleavage assay
• D) High-density oligonucleotide array

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Definitions:

Nicotine

Nicotine is a stimulant drug found in tobacco plants that affects the nervous system and is both addictive and potentially harmful.

Unborn Children

Refers to fetuses or embryos that are in the developmental stages within the womb, prior to birth.

Prenatal Development

The process of growth and development inside the womb, from conception to birth, encompassing all phases of embryonic and fetal development.

Zygote

The initial cell formed when a new organism is conceived, resulting from the union of an ovum and a sperm cell.