Which of the Following Steps Can Be Taken to Minimize

question 9

Multiple Choice

Which of the following steps can be taken to minimize mis-priming in a PCR procedure?

• A) Use high-quality deoxynucleotides, buffers, and enzymes.
• B) Seclude the polymerase from the template and primers before amplification.
• C) Load the samples carefully for gel electrophoresis of PCR products.
• D) Keep the reaction mix at room temperature prior to amplification.

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Definitions:

Otto Von Bismarck

A Prussian statesman who engineered the unification of Germany in 1871 and served as its first Chancellor.

German State

Refers to the political and geographic entity located in Central Europe, known for its rich history, culture, and significant role in both world wars.

Secular

Pertaining to worldly things or to things that are not regarded as religious, spiritual, or sacred; not affiliated with any religious denomination.

Piedmont-Sardinia

A kingdom in northern Italy that played a central role in the unification of Italy in the 19th century.