A Southern Blot Procedure Has Been Performed, but the Molecular

question 1

Multiple Choice

A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?

• A) Decrease the incubation temperature.
• B) Decrease the salt concentration.
• C) Increase the formamide concentration.
• D) Increase the incubation temperature.

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Definitions:

Electronic Control

The use of electronic systems to regulate the operation of mechanical devices, commonly found in automotive, industrial, and consumer appliance contexts.

Sampling

The process of selecting a subset of individuals from a statistical population to estimate characteristics of the whole population.

Significance Level

The chance of incorrectly dismissing the null hypothesis in a statistical examination, usually symbolized by alpha, when it is in fact correct.

Actual Temperatures

Measurements of the current temperature of an environment, object, or substance.