A Researcher Inserts a DNA Segment at the BamHI Recognition

question 157

Short Answer

A researcher inserts a DNA segment at the BamHI recognition site within a plasmid; this site is located within the ampicillin resistance gene.This plasmid also has a gene for tetracycline resistance.Following DNA transformation, the researcher must differentiate the bacteria that have taken up the recombinant DNA from those that have taken up either the DNA segment only or intact plasmids.This experiment uses _______ (number) selectable marker(s).

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Definitions:

Traumatic Event

An emotionally distressing or life-threatening experience that can have lasting effects on an individual's mental and physical well-being.

Intense Response

A strong or extreme reaction to a particular stimulus or event.

Type A Personality

A temperament characterized by high levels of competitiveness, self-imposed stress, and a constant sense of urgency.

Heart Disease

A broad term for a range of diseases affecting the heart, including coronary artery disease, heart rhythm problems, and heart defects among others.