Reverse Transcriptase and Terminal Deoxynucleotidyl Transferase Are Used in Gene

question 27

Multiple Choice

Reverse transcriptase and terminal deoxynucleotidyl transferase are used in gene cloning to

• A) remove single stranded DNA and repair the restriction enzyme site after transformation respectively.
• B) make cDNA and create sticky ends on the cDNA and the staggered ends of the cleaved restriction site.
• C) repair the restriction enzyme site before transformation.
• D) repair the restriction enzyme site after transformation.
• E) remove the sticky ends prior to the DNA ligase step.

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