To Position a Gene in an Expression Vector Downstream from a Promoter

question 37

Multiple Choice

To position a gene in an expression vector downstream from a promoter (i.e., perform directional cloning) , it is best to:

• A) use the largest plasmid available.
• B) treat the gene with DNase I.
• C) restrict both the gene and the plasmid with one restriction endonuclease and then screen all colonies for those that express the gene.
• D) restrict each end of the DNA fragment (gene) with a different restriction endonuclease and do likewise for the plasmid.
• E) use a vector with two sites for the same restriction endonuclease.

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