To Position a Gene in an Expression Vector Downstream from a Promoter

question 37

Multiple Choice

To position a gene in an expression vector downstream from a promoter (i.e., perform directional cloning) , it is best to:

• A) use the largest plasmid available.
• B) treat the gene with DNase I.
• C) restrict both the gene and the plasmid with one restriction endonuclease and then screen all colonies for those that express the gene.
• D) restrict each end of the DNA fragment (gene) with a different restriction endonuclease and do likewise for the plasmid.
• E) use a vector with two sites for the same restriction endonuclease.

Be aware of current technological trends and their societal implications, such as smartphone ownership.

Evaluate the role of media users in processing and sharing information.

Identify the dynamic interplay between structure and agency in shaping social life.

Recognize the collaborative dynamics between elements such as technology, social world, and media in promoting social causes.

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Definitions:

Financing Activities

Transactions and events where funds are raised for the company, which may include issuing stocks, bonds, or taking out loans, affecting the equity and debt of the business.

Cash Flow

A measurement of the net amount of cash and cash-equivalents being transferred into and out of a business.

Common Stock

A form of corporate equity ownership, a type of security representing ownership in a corporation and a claim on part of the corporation's profits.

Statement of Cash Flows

A financial overview showing the cumulative cash inflows from a company's daily operations and investment receipts, against the total cash outlays for operational and investment expenditures during a nominated period.