You Isolate Superoxide Dismutase from Two Cell Culture Lines

question 10

Essay

You isolate superoxide dismutase from two cell culture lines.One of the lines (SOD1)has a level of SOD activity similar to that found in liver,the tissue from which the cell line was originally obtained.The other cell line (SOD10)has elevated SOD activity.The enzyme in SOD10 is extremely efficient at converting the superoxide free radical to hydrogen peroxide.In a routine check of other critical enzyme activities,catalase was found to have activity levels that were severely depressed in SOD10,while they appeared normal in SOD1.Observations of SOD10 reveal that this cell line cannot be maintained as easily as SOD1.SOD10 cells appear to die at an accelerated rate.What,if anything,can you conclude from these data?

Identify correct procedures for vein selection and preparation for IV placement.

Calculate IV infusion rates and durations accurately.

Assess contraindications for IV placement in various clinical scenarios.

Recognize signs and symptoms of fluid overload in patients receiving blood transfusions.

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Definitions:

Vitamin C

A water-soluble vitamin found in various foods, necessary for the growth, development, and repair of all body tissues, and functioning as an antioxidant.

Cyanocobalamin

is a synthetic form of vitamin B12 used to treat and prevent B12 deficiency and related conditions.

Folic Acid

A water-soluble B vitamin that is crucial in the synthesis of nucleic acid and the formation of red blood cells, often recommended during pregnancy.

DNA

A nucleic acid that contains the genetic information of cells.