Examlex

Solved

A Mutant B Cell Line Is Examined by Confocal Microscopy θ\theta

question 39

Multiple Choice

A mutant B cell line is examined by confocal microscopy after incubation with a microbial pathogen recognized by the BCR on these B cells. The B cells have been stained with antibodies to visualize the localization of polymerized actin and microtubules. As a control, wild-type B cells are examined. The results are shown in Figure, with the numbers indicating the proportion of cells examined that show each pattern of staining.  A mutant B cell line is examined by confocal microscopy after incubation with a microbial pathogen recognized by the BCR on these B cells. The B cells have been stained with antibodies to visualize the localization of polymerized actin and microtubules. As a control, wild-type B cells are examined. The results are shown in Figure, with the numbers indicating the proportion of cells examined that show each pattern of staining.     To identify the specific signaling defect in these mutant B cells, a reasonable biochemical assay would be to: A)  Determine if BCR stimulation of mutant B cells produces enhanced binding of the B cell to the microbe B)  Determine whether the mutant B cells have reduced levels of the enzyme Protein kinase C- \theta  C)  Determine whether the mutant B cells are overexpressing the enzyme Vav D)  Determine whether BCR stimulation of mutant B cells promotes exchange of GDP for GTP on cdc42 E)  Determine whether BCR stimulation of mutant B cells produces increased levels of DAG
To identify the specific signaling defect in these mutant B cells, a reasonable biochemical assay would be to:


Definitions:

Crowding out

A situation where increased government spending leads to reduced investment in the private sector due to higher interest rates or competition for resources.

Government Purchases

Expenditures by government entities on goods and services that are used to provide public services or to benefit the country's economy.

Federal Reserve

The central banking system of the United States, responsible for monetary policy, regulation of the banking industry, and stability of the financial system.

Interest Rates

The cost of borrowing money or the return on invested capital, expressed as a percentage of the principal, affecting economic activity by influencing spending and saving behaviors.

Related Questions