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A Site-Directed Mutagenesis Converting Histidine-57 of Chymotrypsin to a Lysine

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Essay

A site-directed mutagenesis converting histidine-57 of chymotrypsin to a lysine results in an inactive enzyme even though lysine has an amino group in its side chain. Describe why the scientist may have thought this result surprising and why it wasn't.


Definitions:

Measured Syringe Volume

The specific amount of liquid, usually medication, that is drawn into a syringe for administration.

Units

Basic quantities used to specify measurements in various fields, such as length, mass, and time.

Diameter

A straight line passing from side to side through the center of a body or figure, especially a circle or sphere.

Gauge

A measure of the thickness or size of various items, like wire or body piercing jewelry, or a device for measurement.

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