You Measure the Initial Velocity of an Enzyme in the Absence

question 19

Essay

You measure the initial velocity of an enzyme in the absence and presence of two inhibitors. In each case, the inhibitor is at 10 µM. Shown in the table below is the primary data for all three cases. Construct a Lineweaver-Burk plot for each case. Calculate the KM and Vmax for each case, both graphically and mathematically. Determine the mechanism for each inhibitor and where each will interact on the enzyme.
Initial Velocity (µmol/ml min)
Enzyme Enzyme Enzyme
[S] mM Alone + Inhibitor 1 + Inhibitor 2
0.33 1.65 1.05 0.79
0.50 2.13 1.43 1.02
1.00 2.99 2.22 1.43
2.00 3.72 3.08 1.79
5.00 4.00 3.80 2.00

---

Definitions:

Short-term Memory

The capacity for holding a small amount of information in an active, readily available state for a short period of time.

Long-term Memory

A type of memory that stores information for a long period, potentially for a lifetime, encompassing experiences, knowledge, and skills.

One-Fifth

Quantitatively, a fraction equivalent to 20 percent or one out of five parts.

Mammals

A class of warm-blooded vertebrate animals that have, in the female, mammary glands which produce milk for feeding their young.