A Site Directed Mutagenesis Converting Histidine 57 of Chymotrypsin to a Lysine

question 48

Essay

A site directed mutagenesis converting histidine 57 of chymotrypsin to a lysine results in an inactive enzyme even though lysine has an amino group in its side chain. Describe why the scientist may have thought this result surprising and why it wasn't.

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Definitions:

Quasi-Endowment Funds

Funds set aside by an organization, often a nonprofit or educational institution, to function like an endowment but which can be accessed under certain conditions, as determined by its governing body.

Operating Reserves

Financial assets set aside by an organization to cover unexpected shortfalls or emergencies, ensuring financial stability and operational continuity.

Permanently Restricted Endowment

A type of endowment fund that a donor restricts in perpetuity; the principal cannot be spent, but the investment earnings can be used for designated purposes.

Financial Assets

Assets that derive value because of a contractual claim, such as stocks, bonds, and bank deposits.