What Is the Most Logical Sequence of Steps for Splicing

question 3

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I.Transform bacteria with a recombinant DNA molecule.
II.Cut the plasmid DNA using restriction enzymes (endonucleases) .
III.Extract plasmid DNA from bacterial cells.
IV.Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V.Use ligase to seal plasmid DNA to non-plasmid DNA.

• A) II, III, V, IV, I
• B) III, II, IV, V, I
• C) III, IV, V, I, II
• D) IV, V, I, II, III

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Definitions:

Memories

Memories are the mental storage of past experiences, knowledge, and sensations, which are capable of influencing present behavior and attitudes.

Forgiveness Process

A psychological and emotional journey that involves letting go of resentment, anger, and thoughts of revenge towards someone who has caused harm.

Offer Forgiveness

The act of pardoning someone for an offense or mistake, thus letting go of resentment.

Forgiveness Misconception

Forgiveness Misconception refers to the false beliefs or misunderstandings about the nature and process of forgiving, such as thinking forgiveness means forgetting or excusing harmful behavior.