You Identify an RFLP in Mice by Digesting Genomic DNA

question 62

Multiple Choice

You identify an RFLP in mice by digesting genomic DNA with HindIII enzyme and radiolabeling a piece of probe DNA.Southern blot analysis shows that the probe detects a 2000 bp fragment in one strain of mice and a 4-kb fragment in another strain of mice.The two mice strains are crossed to produce an F₁ generation.Two F₁ siblings are mated to produce a dozen F₂ progeny.You isolate genomic DNA from several F₁ and F₂ individuals, digest with HindIII, and perform a Southern blot with the same probe. How many bands would be seen in the Southern blot of an F₁ individual?

• A)1
• B)2
• C)3
• D)4
• E)Two of the answers are possible.

---

Definitions:

Dried Up Lake Bed

The base of a former lake that has lost its water content and become dry, often leaving behind salt flats, minerals, or arid land.

Clastic Sediments

Sediments made up of fragments, or clasts, of pre-existing minerals and rock that have been transported and deposited by wind, water, or ice.

Amount Of Sorting

The degree to which sediment particles are of uniform size, which influences the texture and porosity of sedimentary rocks.

Climate

A pattern of weather conditions in an area over a long period of time.