You Are Trying to Determine Whether a Certain RFLP Marker

question 31

Essay

You are trying to determine whether a certain RFLP marker is linked to a specific disease gene in dogs.A cross between a diseased male and a healthy female produces nine offspring.You isolate genomic DNA from parents and offspring, digest with EcoRI, radiolabel a portion of RFLP marker to use as a probe, and perform a Southern analysis.The pedigree and autoradiogram results are shown in the following figure.Assume that the disease is an autosomal dominant. a.Explain the relationship between the crossing results (i.e., diseased versus healthy)and the RFLP patterns.
b.How would you explain the results seen for the second male puppy?

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Definitions:

Beck's Theory

A theory developed by Aaron T. Beck that primarily focuses on the role of negative cognitive biases and thoughts in the development and maintenance of depression.

Prevailing Schemas

Dominant cognitive frameworks or beliefs that influence how individuals interpret and respond to information.

Personality Disorders

Mental health disorders characterized by enduring maladaptive patterns of behavior, cognition, and inner experience, exhibited across many contexts and deviating markedly from those accepted by the individual's culture.

Gender Differences

The distinctions in characteristics, behaviors, roles, and expectations typically associated with being male or female in a given society.