You Set Up an EcoRI Restriction Digest for a Sample

question 13

Multiple Choice

You set up an EcoRI restriction digest for a sample of genomic DNA from an individual with a mutation in the β-globin gene. You perform a Southern blot using the entire β-globin gene as a probe. To your surprise, you notice a different pattern of bands in the affected individual - you have identified a new SNP! What can you conclude from the pattern of bands from the Southern blot below?

• A)The SNP resulted in the loss of an EcoRI site.
• B)The SNP resulted in the creation of a new EcoRI site.
• C)The SNP resulted in the loss of an exon.
• D)The SNP results in the creation of a new HindIII site.
• E)There was no change in the genomic DNA sequence.

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Definitions:

Enabling Others to Act

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Interprofessional Team

A group of healthcare professionals from various disciplines working collaboratively to deliver comprehensive care.

Oncologist

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Aesthetic Knowing

Refers to the nurses' ability to perceive the nuances of patients' experiences and conditions, guiding compassionate and empathetic care.