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Two Probes, a and B, Hybridize Very near Each Other

question 33

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Two probes, A and B, hybridize very near each other in a region of DNA that contains RFLPs when digested with HindIII. The four maps show the location and intervening distances of the HindIII restriction sites and the binding locations of probes A and B. The maps show alleles H1-H4 present in a population. What band(s) is/are seen in Southern blot analysis of a H2H3 heterozygote using probe B? Two probes, A and B, hybridize very near each other in a region of DNA that contains RFLPs when digested with HindIII. The four maps show the location and intervening distances of the HindIII restriction sites and the binding locations of probes A and B. The maps show alleles H<sup>1</sup>-H<sup>4</sup> present in a population. What band(s) is/are seen in Southern blot analysis of a H<sup>2</sup>H<sup>3</sup> heterozygote using probe B?   A) 3 kb and 5 kb B) 3 kb and 8 kb C) 3kb, 2kb, and 8 kb D) 5 kb and 8 kb E) 2 kb, 3 kb, and 5 kb


Definitions:

Hemodialysis

A medical procedure that filters waste and excess fluids from the blood when the kidneys are not functioning properly.

Osmosis

A process by which molecules of a solvent tend to pass through a semipermeable membrane from a less concentrated solution into a more concentrated one, thus equalizing the concentrations on each side of the membrane.

Diffusion

The process by which molecules spread from areas of high concentration to areas of low concentration until there is an even distribution.

Healthy Bowel Elimination

The process of passing waste through and out of the bowel in a manner considered normal and healthy.

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