Two Different Enzymes Are Able to Catalyze the Same Reaction $\rightarrow$

question 47

Short Answer

Two different enzymes are able to catalyze the same reaction, A $\rightarrow$ B. They both have the same V_max, but differ their K_m the substrate A. For enzyme 1, the K_m is 1.0 mM; for enzyme 2, the K_m is 10 mM. When enzyme 1 was incubated with 0.1 mM A, it was observed that B was produced at a rate of 0.0020 mmoles/minute. a) What is the value of the V_max of the enzymes? b) What will be the rate of production of B when enzyme 2 is incubated with 0.1 mM A? c) What will be the rate of production of B when enzyme 1 is incubated with 1 M (i.e., 1000 mM) A?