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A biochemist obtains the following set of data for an enzyme that is known to follow Michaelis-Menten kinetics.
Substrate
Initial
concentration
velocity
( M)
( mol/min)
1
49
2
96
8
349
50
621
100
676
1,000
698
5,000
699
(a) Vmax for the enzyme is _____. Explain in one sentence how you determined Vmax.
(b) Km for the enzyme is _____. Explain in one sentence how you determined Km.
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