The Turnover Number for an Enzyme Is Known to Be $\rightarrow$

question 32

Essay

The turnover number for an enzyme is known to be 5000 min^-1. From the following set of data, calculate the K_m and the total amount of enzyme present in these experiments.
Substrate
Initial
concentration
velocity
(mM)
( $\rightarrow$ mol/min)
1
167
2
250
4
334
6
376
100
498
1,000
499
(a) K_m = _____. (b) Total enzyme = _____ $\mu$ mol.

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