In E Coli Replication, DNA Gyrase Does Not Require ATP Hydrolysis

question 84

Short Answer

In E. coli replication, DNA gyrase does not require ATP hydrolysis to lower the linking number of its substrate. Why is this, and where does the energy come from?

Grasp the principles and methods involved in making transformations in genetic engineering, such as electroporation.

Recognize factors influencing gene expression in various host cell types.

Identify the role of essential tools in genetic engineering, such as plasmids, vectors, and DNA ligase.

Understand the purpose and process of recombinant DNA technology and gene cloning.