In the Diagram Below, the Horizontal Lines Represent DNA Strands

question 162

Multiple Choice

In the diagram below, the horizontal lines represent DNA strands in a double-stranded molecule, the vertical lines mark the positions of cleavage sites for a particular restriction enzyme, the arrows show the positions of primers used in the polymerase chain reaction to amplify the region, and the numbers are the number of nucleotides between successive restriction sites. Suppose that the population contains a restriction fragment length polymorphism (RFLP) for this region of DNA. Some DNA molecules contain the site Y, whereas other DNA molecules lack this site. If we use the symbol Y⁺ to denote the allele containing the Y site and Y^- to denote the allele lacking it, then what bands would be observed in the heterozygous genotype Y⁺/ Y^- after amplification and cleavage with the restriction enzyme?

• A)5 kb
• B)5 kb and 3 kb
• C)5 kb, 3 kb, and 2 kb
• D)3 kb and 2 kb
• E)None of the answer options is correct.

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