What kind of reactive intermediate is present in the mechanism of the following reaction? 
Definitions:
Lagging Strand
The lagging strand is one of the strands of DNA formed during DNA replication, synthesized discontinuously in segments known as Okazaki fragments, opposite the continuous leading strand.
Transcription
The process by which the information in a strand of DNA is copied into a new molecule of messenger RNA (mRNA), initiating gene expression.
Translation
The process in which cellular ribosomes create proteins, mapping instructions from mRNA to synthesize the specific protein.
Amino Acids
Organic compounds that combine to form proteins, serving as the building blocks of life.
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