With Respect to Nucleic Acid Hybridization, Which, If Any, of the Following

question 6

Multiple Choice

With respect to nucleic acid hybridization, which, if any, of the following statements is false?

• A) The strength of base pairing between a probe and a complementary target sequence depends on the number of stable base pairs that are formed.
• B) Among other parameters, the hybridization stringency depends on the salt concentration and temperature of the hybridization reaction.
• C) To identify a target sequence that is distantly related to the probe, high stringency hybridization needs to be used.
• D) Under conditions that favor low hybridization stringency long heteroduplexes with significant base mismatching may be stable.

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