You Want to Design a Set of PCR Primers to Amplify

question 31

Multiple Choice

You want to design a set of PCR primers to amplify a portion of a gene from a cDNA library.Which of the following concerns about PCR primer design is the most legitimate?

• A)You must be careful when designing your primers to take into account which DNA strand was transcribed in mRNA and make sure both primers are complementary to the mRNA.
• B)You must be certain not to include any DNA sequences in your primer that are upstream (5′) of the AUG start codon.
• C)You must make sure that all the DNA sequences in your primer lie within an exon, and do not span two exons.
• D)You must be certain that all the DNA sequences in your primer are not located downstream (3′) of the polyadenylation signal.

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